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犬表皮生长因子受体2(EGFR2)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK0537
  • Price:Negotiable
  • Clicks:2382

Product Detail

Product Information

Product NameDog EGFR2(Epidermal Growth Factor Receptor 2) ELISA Kit
Alternative NamesCD340; ErbB2; HER-2/neu; HER2; NEU; MLN 19; NGL; TKR1; p185; V-Erb-B2 Erythroblastic Leukemia Viral Oncogene Homolog 2; Neuro/Glioblastoma Derived Oncogene Homolog

Assay Parameters

SpeciesDog
Assay TypeSandwich
Sensitivity0.3 ng/mL
Detection Range0.78-50 ng/mL
Sample Typeserum, plasma, tissue homogenates and other biological fluids
Assay Length3.5h

Research Information

Research AreaSignal transduction;Tumor immunity;Reproductive science;Hepatology;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog EGFR2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog EGFR2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog EGFR2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog EGFR2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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