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一步法大鼠孕酮(PG)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK047ES
  • Price:USD $ 2,800.00
  • Clicks:2572

Product Detail

Product Information

Product NameEasyStep Rat PG(Progesterone) ELISA Kit
Alternative NamesP4; Pregn-4-Ene-3,20-Dione

Specifications & Pricing

48T Price1960
96T Price2800
96T*5 Price11900

Assay Parameters

SpeciesRat
Assay TypeCompetitive Inhibition
Sensitivity0.7 ng/mL
Detection Range1.56-100 ng/mL
Sample TypeSerum, plasma
Assay Length1.5h

Research Information

Research AreaEndocrinology;Reproductive science;Hormone metabolism;
Test PrincipleThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat PG antigen, and the Rat PG standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Rat PG to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Rat PG in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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