小鼠信号素6B(SEMA6B)酶联免疫吸附检测试剂盒
- Cat. No.：ELK0455
- Price：Negotiable
- Clicks：2835
Product Detail
Product Information
| Product Name | Mouse SEMA6B(Semaphorin 6B) ELISA Kit |
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| Alternative Names | Semaphorin VIB, Semaphorin-N, Sema VIB, Sema N, Seman |
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Assay Parameters
| Species | Mouse |
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| Assay Type | Sandwich |
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| Sensitivity | 0.054 ng/mL |
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| Detection Range | 0.16-10 ng/mL |
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| Sample Type | tissue homogenates, cell lysates and other biological fluids |
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| Assay Length | 3.5h |
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Research Information
| Research Area | CD & Adhesion molecule; |
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| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse SEMA6B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse SEMA6B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse SEMA6B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse SEMA6B in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Standard Curve
| Standard Curve |  |
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