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小鼠胰淀素(IAPP)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK0441
  • Price:Negotiable
  • Clicks:2537

Product Detail

Product Information

Product NameMouse IAPP(Islet Amyloid Polypeptide) ELISA Kit
Alternative NamesIAP; DAP; Amylin; Insulinoma Amyloid Polypeptide; Diabetes-associated peptide

Assay Parameters

SpeciesMouse
Assay TypeSandwich
Sensitivity2.8 pg/mL
Detection Range7.82-500 pg/mL
Sample TypeSerum, plasma and other biological fluids.
Assay Length3.5h

Research Information

Research AreaMetabolic pathway;Endocrinology;Hepatology;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IAPP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IAPP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IAPP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IAPP in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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