豚鼠免疫球蛋白E(IgE)酶联免疫吸附检测试剂盒
- Cat. No.：ELK0421
- Price：Negotiable
- Clicks：2231
Product Detail
Product Information
| Product Name | Guinea pig IgE(Immunoglobulin E) ELISA Kit |
|---|---|
| Alternative Names | IGHE; Immunoglobulin Heavy Constant Epsilon; Ig epsilon chain C region |
Assay Parameters
| Species | Guinea pig |
|---|---|
| Assay Type | Sandwich |
| Sensitivity | 31.7 ng/mL |
| Detection Range | 93.75-6000 ng/mL |
| Sample Type | Serum, plasma and other biological fluids |
| Assay Length | 3.5h |
Research Information
| Research Area | Infection immunity;Immune molecule;Hematology;Autoimmunity; |
|---|---|
| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Guinea pig IgE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Guinea pig IgE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Guinea pig IgE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Guinea pig IgE in the samples is then determined by comparing the OD of the samples to the standard curve. |
Standard Curve
| Standard Curve |  |
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