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小鼠B、T淋巴细胞衰减因子(BTLA)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK0419
  • Price:Negotiable
  • Clicks:2732

Product Detail

Product Information

Product NameMouse BTLA(B- and T-lymphocyte attenuator) ELISA Kit
Alternative NamesB and T lymphocyte associated protein; B and T lymphocyte attenuator; B and T lymphocyte associated; B- and T-lymphocyte attenuator; B- and T-lymphocyte-associated protein; BTLA; BTLA1; CD272; CD272 antigen

Assay Parameters

SpeciesMouse
Assay TypeSandwich
Sensitivity0.21 ng/mL
Detection Range0.63-40 ng/mL
Sample TypeSerum, plasma, urine, tissue homogenates, cell culture supernates and other biological fluids
Assay Length3.5h

Research Information

Research AreaCardiac function; Cancer
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse BTLA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse BTLA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse BTLA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse BTLA in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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