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人磷酸葡萄糖变位酶5(PGM5)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK0406
  • Price:USD $ 2,800.00
  • Clicks:2616

Product Detail

Product Information

Product NameHuman (Phosphoglucomutase-like protein 5) ELISA Kit
Alternative NamesPGMRP

Specifications & Pricing

48T Price1960
96T Price2800
96T*5 Price11900

Assay Parameters

SpeciesHuman
Assay TypeSandwich
Sensitivity0.12 ng/mL
Detection Range0.32-20 ng/mL
Sample TypeTissue homogenates and other biological fluids.
Assay Length3.5h

Research Information

Research AreaSignal transduction; Neuro science
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PGM5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PGM5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PGM5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PGM5 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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