小鼠RAD51同源物(RAD51)酶联免疫吸附检测试剂盒
- Cat. No.：ELK0402
- Price：Negotiable
- Clicks：2332
Product Detail
Product Information
| Product Name | Mouse RAD51(RAD51 Homolog) ELISA Kit |
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| Alternative Names | RAD51A; RECA; HsRad51; BRCC5; BRCA1/BRCA2-Containing Complex Subunit 5; DNA repair protein RAD51 homolog 1 |
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Assay Parameters
| Species | Mouse |
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| Assay Type | Sandwich |
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| Sensitivity | 0.05 ng/mL |
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| Detection Range | 0.16-10 ng/mL |
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| Sample Type | tissue homogenates, cell lysates and other biological fluids |
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| Assay Length | 3.5h |
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Research Information
| Research Area | Signal transduction; |
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| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse RAD51. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse RAD51. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse RAD51, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse RAD51 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Standard Curve
| Standard Curve |  |
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