小鼠核心蛋白聚糖(DCN)酶联免疫吸附检测试剂盒
- Cat. No.：ELK0400
- Price：Negotiable
- Clicks：3003
Product Detail
Product Information
| Product Name | Mouse DCN(Decorin) ELISA Kit |
|---|---|
| Alternative Names | CSCD; DSPG2; PG40; PGII; PGS2; PG-S2; SLRR1B; Bone proteoglycan II |
Assay Parameters
| Species | Mouse |
|---|---|
| Assay Type | Sandwich |
| Sensitivity | 6.36 pg/mL |
| Detection Range | 15.63-1000 pg/mL |
| Sample Type | serum, plasma, tissue homogenates and other biological fluids |
| Assay Length | 3.5h |
Research Information
| Research Area | Signal transduction;Developmental science;Bone metabolism; |
|---|---|
| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse DCN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse DCN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse DCN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse DCN in the samples is then determined by comparing the OD of the samples to the standard curve. |
Standard Curve
| Standard Curve |  |
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