小鼠含Patatin样磷脂酶域蛋白2(PNPLA2)微量上样酶联免疫吸附检测试剂盒
- Cat. No.：ELK0382MS
- Price：Negotiable
- Clicks：2206
Product Detail
Product Information
| Product Name | Mouse PNPLA2(Patatin Like Phospholipase Domain Containing Protein 2) Microsample ELISA Kit |
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| Alternative Names | ATGL; PEDF-R; TTS2; Desnutrin; Pigment epithelium-derived factor; Transport-secretion protein 2; Adipose Triglyceride Lipase; Calcium-independent phospholipase A2 |
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Assay Parameters
| Species | Mouse |
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| Assay Type | Sandwich |
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| Sensitivity | 0.062 ng/mL |
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| Detection Range | 0.16-10 ng/mL |
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| Sample Type | tissue homogenates, cell lysates and other biological fluids |
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| Assay Length | 3.5h |
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Research Information
| Research Area | Signal transduction; |
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| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PNPLA2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PNPLA2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PNPLA2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PNPLA2 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Standard Curve
| Standard Curve |  |
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