小鼠转录激活因子4(ATF4)酶联免疫吸附检测试剂盒
- Cat. No.：ELK0365
- Price：Negotiable
- Clicks：2655
Product Detail
Product Information
| Product Name | Mouse ATF4(Activating Transcription Factor 4) ELISA Kit |
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| Alternative Names | TAXREB67; TXREB; CREB-2; DNA-binding protein TAXREB67; Cyclic AMP-responsive element-binding protein 2; Tax-responsive enhancer element-binding protein 67 |
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Assay Parameters
| Species | Mouse |
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| Assay Type | Sandwich |
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| Sensitivity | 30.4 pg/mL |
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| Detection Range | 78.13-5000 pg/mL |
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| Sample Type | Tissue homogenates, cell lysates and other biological fluids. |
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| Assay Length | 3.5h |
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Research Information
| Research Area | Signal transduction;Developmental science; |
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| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ATF4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ATF4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ATF4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ATF4 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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| UniProt ID | Q06507 |
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Standard Curve
| Standard Curve |  |
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