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人含有半胱天冬酶募集域蛋白9(CARD9)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK0362
  • Price:Negotiable
  • Clicks:2085

Product Detail

Product Information

Product NameHuman CARD9(Caspase Recruitment Domain-Containing Protein 9) ELISA Kit
Alternative NamesCARD9 ; CANDF2; Caspase recruitment domain family member 9 ; Caspase recruitment domain-containing protein 9 ; hCARD9

Assay Parameters

SpeciesHuman
Assay TypeSandwich
Sensitivity0.077 ng/mL
Detection Range0.16-10 ng/mL
Sample TypeSerum, plasma and other biological fluids
Assay Length3.5h

Research Information

Research AreaImmunology
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CARD9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CARD9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CARD9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CARD9 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDQ9H257

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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