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一步法人氨基端前脑钠素(NT-ProBNP)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK035ES
  • Price:Negotiable
  • Clicks:1801

Product Detail

Product Information

Product NameEasyStep Human NT-ProBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit
Alternative NamesNT-Pro-BNP; N-BNP; ANFB_HUMAN; BNP(1-32); BNP(5-29); BNP-32; Gamma-brain natriuretic peptide; nppb

Assay Parameters

SpeciesHuman
Assay TypeSandwich
Sensitivity0.94 ng/mL
Detection Range0.94-60 ng/mL
Sample TypeSerum, plasma
Assay Length1.5h

Research Information

Research AreaEndocrinology;Cardiovascular biology;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NT-ProBNP, and the Human NT-ProBNP standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human NT-ProBNP. After TMB substrate solution is added, only those wells that contain Human NT-ProBNP and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NT-ProBNP in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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