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大鼠c-Jun氨基末端激酶(JNK)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK0354
  • Price:USD $ 2,800.00
  • Clicks:2010

Product Detail

Product Information

Product NameRat JNK(C-Jun N-Terminal Kinases) ELISA Kit
Alternative NamesMAPK8; JNK; JNK1; PRKM8; SAPK1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2; c-Jun N-terminal kinase JNK

Specifications & Pricing

48T Price1960
96T Price2800
96T*5 Price11900

Assay Parameters

SpeciesRat
Assay TypeSandwich
Sensitivity0.14 ng/mL
Detection Range0.32-20 ng/mL
Sample Typetissue homogenates, cell lysates and other biological fluids
Assay Length3.5h

Research Information

Research AreaEnzyme & Kinase;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat JNK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat JNK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat JNK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat JNK in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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