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大鼠线粒体融合素2(MFN2)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK0352
  • Price:Negotiable
  • Clicks:2454

Product Detail

Product Information

Product NameRat MFN2(Mitofusin 2) ELISA Kit
Alternative NamesCMT2A; CMT2A2; CPRP1; HSG; MARF; Transmembrane GTPase MFN2

Assay Parameters

SpeciesRat
Assay TypeSandwich
Sensitivity0.071 ng/mL
Detection Range0.16-10 ng/mL
Sample Typetissue homogenates, cell lysates and other biological fluids
Assay Length3.5h

Research Information

Research AreaMetabolic pathway;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MFN2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MFN2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MFN2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MFN2 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDQ8R500

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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