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人窖蛋白3(CAV3)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK0347
  • Price:Negotiable
  • Clicks:2585

Product Detail

Product Information

Product NameHuman CAV3(Caveolin 3) ELISA Kit
Alternative NamesM-caveolin

Assay Parameters

SpeciesHuman
Assay TypeSandwich
Sensitivity0.064 ng/mL
Detection Range0.16-10 ng/mL
Sample Typetissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length3.5h

Research Information

Research AreaTumor immunity;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CAV3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CAV3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CAV3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CAV3 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDP56539

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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