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小鼠细胞胱硫醚γ裂解酶(CSE)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK0337
  • Price:Negotiable
  • Clicks:2721

Product Detail

Product Information

Product NameMouse CSE(Cystathionine Gamma Lyase) ELISA Kit
Alternative NamesCTH; Cystathionase; Cysteine-protein sulfhydrase; Gamma-cystathionase

Assay Parameters

SpeciesMouse
Assay TypeSandwich
Sensitivity0.11 ng/mL
Detection Range0.32-20 ng/mL
Sample TypeSerum, plasma, tissue homogenates and other biological fluids.
Assay Length3.5h

Research Information

Research AreaEnzyme & Kinase;Metabolic pathway;Cardiovascular biology;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CSE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CSE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CSE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CSE in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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