牛骨特异性碱性磷酸酶(BALP)酶联免疫吸附检测试剂盒
- Cat. No.：ELK0331
- Price：Negotiable
- Clicks：2785
Product Detail
Product Information
| Product Name | Cattle BALP(Bone-specific Alkaline Phosphatase) ELISA Kit |
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| Alternative Names | BALP; BSAP; HOPS; AP-TNAP; TNSALP; Bone Alkaline Phosphatase; Alkaline Phosphatase,Tissue-Nonspecific Isozyme |
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Assay Parameters
| Species | Cattle |
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| Assay Type | Sandwich |
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| Sensitivity | 0.49 ng/mL |
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| Detection Range | 1.57-100 ng/mL |
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| Sample Type | serum, plasma and other biological fluids |
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| Assay Length | 3.5h |
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Research Information
| Research Area | Enzyme & Kinase;Metabolic pathway;Hepatology;Bone metabolism; |
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| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle BALP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle BALP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle BALP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle BALP in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Standard Curve
| Standard Curve |  |
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