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小鼠H2A组蛋白家族成员X(H2AFX)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK0328
  • Price:USD $ 2,800.00
  • Clicks:2065

Product Detail

Product Information

Product NameMouse H2AFX(H2A Histone Family, Member X) ELISA Kit
Alternative NamesH2AX

Specifications & Pricing

48T Price1960
96T Price2800
96T*5 Price11900

Assay Parameters

SpeciesMouse
Assay TypeSandwich
Sensitivity11.6 pg/mL
Detection Range31.25-2000 pg/mL
Sample TypeSerum, plasma, tissue homogenates and other biological fluids.
Assay Length3.5h

Research Information

Research AreaMetabolic pathway;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse H2AFX. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse H2AFX. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse H2AFX, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse H2AFX in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDP27661

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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