人丙酮酸脱氢酶激酶同工酶4(PDK4)酶联免疫吸附检测试剂盒
- Cat. No.：ELK0321
- Price：Negotiable
- Clicks：2015
Product Detail
Product Information
| Product Name | Human PDK4(Pyruvate Dehydrogenase Kinase Isozyme 4) ELISA Kit |
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| Alternative Names | [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial; Pyruvate Dehydrogenase Kinase 4 |
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Assay Parameters
| Species | Human |
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| Assay Type | Sandwich |
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| Sensitivity | 0.071 ng/mL |
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| Detection Range | 0.16-10 ng/mL |
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| Sample Type | Serum, plasma, tissue homogenates and other biological fluids. |
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| Assay Length | 3.5h |
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Research Information
| Research Area | Metabolic pathway;Cardiovascular biology; |
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| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PDK4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PDK4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PDK4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PDK4 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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| UniProt ID | Q16654 |
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Standard Curve
| Standard Curve |  |
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