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人糖类抗原15-3(CA15-3)微量上样酶联免疫吸附检测试剂盒

  • Cat. No.:ELK0245MS
  • Price:USD $ 2,800.00
  • Clicks:2060

Product Detail

Product Information

Product NameHuman CA15-3(Carbonhydrate Antigen 15-3) Microsample ELISA Kit
Alternative NamesCA15-3

Specifications & Pricing

48T Price1960
96T Price2800
96T*5 Price11900

Assay Parameters

SpeciesHuman
Assay TypeSandwich
Sensitivity0.49 U/mL
Detection Range1.57-100 U/mL
Sample Typeserum, plasma and other biological fluids
Assay Length3.5h

Research Information

Research AreaTumor immunity;Infection immunity;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CA15-3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CA15-3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CA15-3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CA15-3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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