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小鼠干扰素调节因子7(IRF7)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK0241
  • Price:Negotiable
  • Clicks:1792

Product Detail

Product Information

Product NameMouse IRF7(Interferon Regulatory Factor 7) ELISA Kit
Alternative NamesInterferon regulatory factor 7; IRF-7; IRF7

Assay Parameters

SpeciesMouse
Assay TypeSandwich
Sensitivity11.2 pg/mL
Detection Range31.25-2000 pg/mL
Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length3.5h

Research Information

Research AreaImmune molecule;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IRF7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IRF7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IRF7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IRF7 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDP70434

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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