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大鼠四旋蛋白30(TSPAN30)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK0227
  • Price:Negotiable
  • Clicks:2057

Product Detail

Product Information

Product NameRat TSPAN30(Tetraspanin 30) ELISA Kit
Alternative NamesCD63; LAMP-3; ME491; MLA1; OMA81H; Melanoma 1 antigen; Granulophysin; Lysosomal-associated membrane protein 3; Ocular melanoma-associated antigen

Assay Parameters

SpeciesRat
Assay TypeSandwich
Sensitivity18.3 pg/mL
Detection Range62.5-4000 pg/mL
Sample Typeserum, plasma, tissue homogenates, cell lysates, breast milk, cell culture supernates and other biological fluids
Assay Length3.5h

Research Information

Research AreaSignal transduction;CD & Adhesion molecule;Hematology;Developmental science;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TSPAN30. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TSPAN30. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TSPAN30, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TSPAN30 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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