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小鼠血小板衍生生长因子D(PDGFD)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK0216
  • Price:Negotiable
  • Clicks:2419

Product Detail

Product Information

Product NameMouse PDGFD(Platelet Derived Growth Factor D) ELISA Kit
Alternative NamesPDGF-D; IEGF; SCDGF-B; Spinal Cord Derived Growth Factor B; Iris-expressed growth factor; Platelet-derived growth factor D, receptor-binding form/latent form

Assay Parameters

SpeciesMouse
Assay TypeSandwich
Sensitivity5.13 pg/mL
Detection Range15.63-1000 pg/mL
Sample Typeserum, plasma and other biological fluids
Assay Length3.5h

Research Information

Research AreaCytokine;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PDGFD. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PDGFD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PDGFD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PDGFD in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDQ925I7

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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