犬氨基端前脑钠素(NT-ProBNP)酶联免疫吸附检测试剂盒
- Cat. No.：ELK0199
- Price：Negotiable
- Clicks：2424
Product Detail
Product Information
| Product Name | Dog NT-ProBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit |
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| Alternative Names | NT-Pro-BNP; N-BNP; ANFB_HUMAN; BNP(1-32); BNP(5-29); BNP-32; Gamma-brain natriuretic peptide; nppb |
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Assay Parameters
| Species | Dog |
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| Assay Type | Sandwich |
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| Sensitivity | 24.8 pg/mL |
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| Detection Range | 78.13-5000 pg/mL |
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| Sample Type | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
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| Assay Length | 3.5h |
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Research Information
| Research Area | Endocrinology;Cardiovascular biology; |
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| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog NT-ProBNP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog NT-ProBNP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog NT-ProBNP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog NT-ProBNP in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Standard Curve
| Standard Curve |  |
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