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马免疫球蛋白G(IgG)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK0194
  • Price:Negotiable
  • Clicks:2247

Product Detail

Product Information

Product NameHorse IgG(Immunoglobulin G) ELISA Kit
Alternative NamesImmunoglobulin G

Assay Parameters

SpeciesHorse
Assay TypeSandwich
Sensitivity0.49 µg/mL
Detection Range1.57-100 µg/mL
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Assay Length3.5h

Research Information

Research AreaInfection immunity;Immune molecule;Hematology;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Horse IgG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse IgG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Horse IgG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse IgG in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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