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小鼠白介素1受体关联激酶3(IRAK3)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK0183
  • Price:Negotiable
  • Clicks:2501

Product Detail

Product Information

Product NameMouse IRAK3(Interleukin 1 Receptor Associated Kinase 3) ELISA Kit
Alternative NamesIRAK-M; IRAKM; IL-1 receptor-associated kinase M

Assay Parameters

SpeciesMouse
Assay TypeSandwich
Sensitivity0.11 ng/mL
Detection Range0.32-20 ng/mL
Sample Typeserum, plasma, tissue homogenates and other biological fluids
Assay Length3.5h

Research Information

Research AreaSignal transduction;Enzyme & Kinase;Tumor immunity;Infection immunity;Hematology;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IRAK3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IRAK3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IRAK3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IRAK3 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDQ8K4B2

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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