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小鼠抑制素βB(INHbB)微量上样酶联免疫吸附检测试剂盒

  • Cat. No.:ELK0172MS
  • Price:Negotiable
  • Clicks:2853

Product Detail

Product Information

Product NameMouse INHbB(Inhibin Beta B) Microsample ELISA Kit
Alternative NamesINH-BB; Activin AB Beta Polypeptide

Assay Parameters

SpeciesMouse
Assay TypeSandwich
Sensitivity4.72 pg/mL
Detection Range15.63-1000 pg/mL
Sample TypeSerum, plasma and other biological fluids
Assay Length3.5h

Research Information

Research AreaTumor immunity;Endocrinology;Reproductive science;Developmental science;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse INHbB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse INHbB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse INHbB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse INHbB in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDQ04999

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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