大鼠胰多肽(PP)微量上样酶联免疫吸附检测试剂盒

Product Name	Rat PP(Pancreatic Polypeptide) Microsample ELISA Kit
Alternative Names	PPY; PNP; Pancreatic Polypeptide Y; Obinepitide; Pancreatic icosapeptide; Pancreatic prohormone

Species	Rat
Assay Type	Sandwich
Sensitivity	8.19 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Type	Serum, plasma and other biological fluids
Assay Length	3.5h

Research Area	Endocrinology;
Test Principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PP in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID	P06303

Standard Curve

For Research Use Only. Not for Diagnostic Procedures.

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