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绵羊转化生长因子β1(TGFb1)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK0154
  • Price:USD $ 2,800.00
  • Clicks:2442

Product Detail

Product Information

Product NameSheep TGFb1(Transforming Growth Factor Beta 1) ELISA Kit
Alternative NamesTGF Beta 1; TGF-B1; CED; DPD1; LAP; Camurati-Engelmann Disease; Latency-associated peptide

Specifications & Pricing

48T Price1960
96T Price2800
96T*5 Price11900

Assay Parameters

SpeciesSheep
Assay TypeSandwich
Sensitivity12.1 pg/mL
Detection Range31.25-2000 pg/mL
Sample Typeserum, platelet-poor plasma, tissue homogenates, cell culture supernates and other biological fluids
Assay Length3.5h

Research Information

Research AreaCytokine;Tumor immunity;Infection immunity;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sheep TGFb1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep TGFb1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sheep TGFb1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sheep TGFb1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDP50414

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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