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硝基酪氨酸(NT)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK0150
  • Price:USD $ 2,800.00
  • Clicks:2150

Product Detail

Product Information

Product NameNT(Nitrotyrosine) ELISA Kit
Alternative NamesNitrotyrosine

Specifications & Pricing

48T Price1960
96T Price2800
96T*5 Price11900

Assay Parameters

SpeciesGeneral
Assay TypeCompetitive Inhibition
Sensitivity2.1 ng/mL
Detection Range7.82-500 ng/mL
Sample Typeserum, plasma and other biological fluids
Assay Length2h

Research Information

Research AreaMetabolic pathway;Infection immunity;Rheumatology;
Test PrincipleThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with NT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to NT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of NT in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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