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马糖化血红蛋白A1c(HbA1c)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK0148
  • Price:USD $ 2,800.00
  • Clicks:2156

Product Detail

Product Information

Product NameHorse HbA1c(Glycated Hemoglobin A1c) ELISA Kit
Alternative NamesGlycosylated Hemoglobin; Hemoglobin A1c; Hb1c; HbAIc; HbAIc

Specifications & Pricing

48T Price1960
96T Price2800
96T*5 Price11900

Assay Parameters

SpeciesHorse
Assay TypeSandwich
Sensitivity4.95 µg/mL
Detection Range15.63-1000 µg/mL
Sample Typeserum, plasma and erythrocyte lysates
Assay Length3.5h

Research Information

Research AreaDiagnosis of diabetes mellitus
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Horse HbA1c. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse HbA1c. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Horse HbA1c, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse HbA1c in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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