人琥珀酸脱氢酶复合体D亚基(SDHD)酶联免疫吸附检测试剂盒
- Cat. No.：ELK0127
- Price：Negotiable
- Clicks：2078
Product Detail
Product Information
| Product Name | Human SDHD(Succinate Dehydrogenase Complex Subunit D) ELISA Kit |
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| Alternative Names | PGL; PGL1; QPs3; Succinate-ubiquinone reductase membrane anchor subunit; Succinate dehydrogenase [ubiquinone] cytochrome b small subunit, mitochondrial |
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Assay Parameters
| Species | Human |
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| Assay Type | Sandwich |
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| Sensitivity | 0.066 ng/mL |
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| Detection Range | 0.16-10 ng/mL |
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| Sample Type | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
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| Assay Length | 3.5h |
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Research Information
| Research Area | Enzyme & Kinase; |
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| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SDHD. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SDHD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SDHD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SDHD in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Standard Curve
| Standard Curve |  |
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