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马超氧化物歧化酶1(SOD1)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK0118
  • Price:Negotiable
  • Clicks:2204

Product Detail

Product Information

Product NameHorse SOD1(Superoxide Dismutase 1, Soluble) ELISA Kit
Alternative NamesALS; ALS1; IPOA; Amyotrophic Lateral Sclerosis 1,Adult; Superoxide dismutase [Cu-Zn]

Assay Parameters

SpeciesHorse
Assay TypeSandwich
Sensitivity0.21 ng/mL
Detection Range0.78-50 ng/mL
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length3.5h

Research Information

Research AreaEnzyme & Kinase;Metabolic pathway;Endocrinology;Hepatology;Nutrition metabolism;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Horse SOD1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse SOD1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Horse SOD1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse SOD1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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