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人4-羟基壬烯酸(HNE)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK0114
  • Price:USD $ 2,600.00
  • Clicks:2588

Product Detail

Product Information

Product NameHuman HNE(4-Hydroxynonenal) ELISA Kit
Alternative Names4-HNE; HNE; 4-hydroxy-2-nonenal; 4-Hydroxynonenal

Specifications & Pricing

48T Price1820
96T Price2600
96T*5 Price11050

Assay Parameters

SpeciesHuman
Assay TypeSandwich
Sensitivity1.21 ng/mL
Detection Range3.13-200 ng/mL
Sample TypeSerum, plasma, tissue homogenates
Assay Length3.5h

Research Information

Research AreaSignal transduction;Metabolic pathway;Infection immunity;Hormone metabolism;Dermatology;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HNE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HNE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HNE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HNE in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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