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牛心肌肌钙蛋白T(cTnT/TNNT2)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK0100
  • Price:Negotiable
  • Clicks:2205

Product Detail

Product Information

Product NameCattle cTnT/TNNT2(Troponin T Type 2, Cardiac) ELISA Kit
Alternative NamesCMH2; cTnT; TnTc; Cardiomyopathy,Hypertrophic 2; Cardiac muscle troponin T; TNNT2; Troponin T Type 2, Cardiac

Assay Parameters

SpeciesCattle
Assay TypeSandwich
Sensitivity26.1 pg/mL
Detection Range78.13-5000 pg/mL
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Assay Length3.5h

Research Information

Research AreaCardiovascular biology;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle cTnT/TNNT2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle cTnT/TNNT2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle cTnT/TNNT2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle cTnT/TNNT2 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDP13789

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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