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大鼠嘌呤能受体P2X7(P2RX7)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK0082
  • Price:USD $ 2,400.00
  • Clicks:1916

Product Detail

Product Information

Product NameRat P2RX7(Purinergic Receptor P2X, Ligand Gated Ion Channel 7) ELISA Kit
Alternative NamesP2X7; P2X Purinoceptor 7; ATP receptor; Purinergic receptor

Specifications & Pricing

48T Price1680
96T Price2400
96T*5 Price10200

Assay Parameters

SpeciesRat
Assay TypeSandwich
Sensitivity0.098 ng/mL
Detection Range0.32-20 ng/mL
Sample Typetissue homogenates, cell lysates and other biological fluids
Assay Length3.5h

Research Information

Research AreaCD & Adhesion molecule;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat P2RX7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat P2RX7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat P2RX7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat P2RX7 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDQ64663

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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