大鼠Smad同源物1(Smad1)酶联免疫吸附检测试剂盒
- Cat. No.：ELK0074
- Price：Negotiable
- Clicks：2413
Product Detail
Product Information
| Product Name | Rat Smad1(Mothers Against Decapentaplegic Homolog 1) ELISA Kit |
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| Alternative Names | BSP1; JV4-1; JV41; MADH1; MADR1; SMAD Family Member 1; Mad-related protein 1; Transforming growth factor-beta-signaling protein 1; Mothers against DPP homolog 1 |
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Assay Parameters
| Species | Rat |
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| Assay Type | Sandwich |
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| Sensitivity | 0.132 ng/mL |
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| Detection Range | 0.32-20 ng/mL |
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| Sample Type | Tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
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| Assay Length | 3.5h |
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Research Information
| Research Area | Developmental science; |
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| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat Smad1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Smad1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat Smad1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat Smad1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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| UniProt ID | P97588 |
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Standard Curve
| Standard Curve |  |
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