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人腺苷A3受体(ADORA3)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK0071
  • Price:Negotiable
  • Clicks:2602

Product Detail

Product Information

Product NameHuman ADORA3(Adenosine A3 Receptor) ELISA Kit
Alternative NamesAA3R; AD026

Assay Parameters

SpeciesHuman
Assay TypeSandwich
Sensitivity0.067 ng/mL
Detection Range0.16-10 ng/mL
Sample TypeSerum, plasma and other biological fluids
Assay Length3.5h

Research Information

Research AreaCardiovascular biology;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ADORA3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ADORA3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ADORA3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ADORA3 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDP0DMS8

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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