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大鼠白三烯B4受体1(LTB4R)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK0068
  • Price:Negotiable
  • Clicks:1885

Product Detail

Product Information

Product NameRat LTB4R(Leukotriene B4 receptor 1) ELISA Kit
Alternative NamesLtb4r1; BLT1; BLT-1; LTB4-R1

Assay Parameters

SpeciesRat
Assay TypeSandwich
Sensitivity0.12 ng/mL
Detection Range0.32-20 ng/mL
Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length3.5h

Research Information

Research AreaMetabolic pathway;Infection immunity;Endocrinology;Hormone metabolism;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat LTB4R. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat LTB4R. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat LTB4R, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat LTB4R in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDQ9R0Q2

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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