人神经肽B(NP-B)酶联免疫吸附检测试剂盒

Product Name	Human NP-B(Neuropeptide B) ELISA Kit
Alternative Names	L7; PPNPB; PPL7; NPB

Species	Human
Assay Type	Sandwich
Sensitivity	26.3 pg/mL
Detection Range	78.13-5000 pg/mL
Sample Type	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length	3.5h

Research Area	Neuro science;Hormone metabolism;
Test Principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NP-B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NP-B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NP-B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NP-B in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID	Q8NG41

Standard Curve

For Research Use Only. Not for Diagnostic Procedures.

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