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仓鼠10kDa干扰素γ诱导蛋白(IP10)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK0055
  • Price:Negotiable
  • Clicks:2906

Product Detail

Product Information

Product NameHamster IP10(Interferon Gamma Induced Protein 10kDa) ELISA Kit
Alternative NamesCXCL10; C7; IFI10; INP10; SCYB10; Crg2; GIP10; Mob1; Chemokine C-X-C Motif Ligand 10; Small Inducible Cytokine Subfamily B(Cys-X-Cys)Member 10

Assay Parameters

SpeciesHamster
Assay TypeSandwich
Sensitivity5.8 pg/mL
Detection Range15.63-1000 pg/mL
Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length3.5h

Research Information

Research AreaCytokine;Tumor immunity;Infection immunity;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hamster IP10. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Hamster IP10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hamster IP10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hamster IP10 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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