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小鼠胶质细胞源性连接蛋白(GDN)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK0036
  • Price:Negotiable
  • Clicks:2605

Product Detail

Product Information

Product NameMouse GDN(Glia Derived Nexin) ELISA Kit
Alternative NamesSERPINE2; PI7; PN1; PNI; Protease Nexin I; Serpin Peptidase Inhibitor Clade E Member 2; Peptidase inhibitor 7; Protease nexin I

Assay Parameters

SpeciesMouse
Assay TypeSandwich
Sensitivity0.065 ng/mL
Detection Range0.16-10 ng/mL
Sample Typeserum, plasma, tissue homogenates and other biological fluids
Assay Length3.5h

Research Information

Research AreaNeuro science;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse GDN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GDN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse GDN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse GDN in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDQ07235

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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