小鼠神经颗粒素(NRGN)酶联免疫吸附检测试剂盒

Product Name	Mouse NRGN(Neurogranin) ELISA Kit
Alternative Names	Ng; NEUG; Protein Kinase C Substrate RC3

Species	Mouse
Assay Type	Sandwich
Sensitivity	0.054 ng/mL
Detection Range	0.16-10 ng/mL
Sample Type	tissue homogenates, cell lysates and other biological fluids
Assay Length	3.5h

Research Area	Neuro science;
Test Principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse NRGN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NRGN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse NRGN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NRGN in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID	P60761

Standard Curve

For Research Use Only. Not for Diagnostic Procedures.

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