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大鼠环AMP元件调节剂(CREM)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK0027
  • Price:USD $ 2,800.00
  • Clicks:2278

Product Detail

Product Information

Product NameRat CREM(cAMP Responsive Element Modulator) ELISA Kit
Alternative NamesInducible cAMP early repressor; ICER

Specifications & Pricing

48T Price1960
96T Price2800
96T*5 Price11900

Assay Parameters

SpeciesRat
Assay TypeSandwich
Sensitivity26.3 pg/mL
Detection Range78.13-5000 pg/mL
Sample TypeSerum, plasma and other biological fluids.
Assay Length3.5h

Research Information

Research AreaSignal transduction;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CREM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CREM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CREM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CREM in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDQ03061

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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