小鼠细胞色素P450家族成员1A2(CYP1A2)酶联免疫吸附检测试剂盒
- Cat. No.：ELK0020
- Price：Negotiable
- Clicks：3015
Product Detail
Product Information
| Product Name | Mouse CYP1A2(Cytochrome P450 1A2) ELISA Kit |
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| Alternative Names | CP12; P3-450; P450(PA); Cytochrome P450,Family 1,Subfamily A,Polypeptide 2 |
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Assay Parameters
| Species | Mouse |
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| Assay Type | Sandwich |
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| Sensitivity | 0.12 ng/mL |
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| Detection Range | 0.32-20 ng/mL |
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| Sample Type | Tissue homogenates, cell lysates and other biological fluids |
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| Assay Length | 3.5h |
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Research Information
| Research Area | Metabolic pathway; |
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| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CYP1A2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CYP1A2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CYP1A2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CYP1A2 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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| UniProt ID | P00186 |
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Standard Curve
| Standard Curve |  |
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