鸡降钙素(CT)酶联免疫吸附检测试剂盒

Product Name	Chicken CT(Calcitonin) ELISA Kit
Alternative Names	CALCA; CALC; Calcitonin carboxyl-terminal peptide; CCP; PDN-21Calcitonin carboxyl-terminal peptide; PDN-21

Species	Chicken
Assay Type	Sandwich
Sensitivity	0.067 ng/mL
Detection Range	0.16-10 ng/mL
Sample Type	tissue homogenates, cell lysates and other biological fluids
Assay Length	3.5h

Research Area	Metabolic pathway;Endocrinology;Hormone metabolism;Bone metabolism;
Test Principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chicken CT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken CT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chicken CT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken CT in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID	P01258

Standard Curve

For Research Use Only. Not for Diagnostic Procedures.

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