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人唾液酸结合Ig样凝集素15(SIGLEC15)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK0014
  • Price:Negotiable
  • Clicks:2780

Product Detail

Product Information

Product NameHuman SIGLEC15(Sialic Acid Binding Ig Like Lectin 15) ELISA Kit
Alternative NamesHsT1361; SIGLEC-15; CD33L3

Assay Parameters

SpeciesHuman
Assay TypeSandwich
Sensitivity0.064 ng/mL
Detection Range0.16-10 ng/mL
Sample Typeserum, plasma, saliva, tears and other biological fluids and other biological fluids
Assay Length3.5h

Research Information

Research AreaInfection immunity;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SIGLEC15. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SIGLEC15. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SIGLEC15, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SIGLEC15 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDQ6ZMC9

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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