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人多个EGF样结构域8(MEGF8)酶联免疫吸附检测试剂盒

  • Cat. No.:ELK0005
  • Price:Negotiable
  • Clicks:3154

Product Detail

Product Information

Product NameHuman MEGF8(Multiple Epidermal Growth Factor-Like Domains Protein 8) ELISA Kit
Alternative NamesC19orf49; CRPT2; EGFL4; SBP1

Assay Parameters

SpeciesHuman
Assay TypeSandwich
Sensitivity0.063 ng/mL
Detection Range0.16-10 ng/mL
Sample Typeserum, plasma, saliva, tears and other biological fluids and other biological fluids
Assay Length3.5h

Research Information

Research AreaSignal transduction;
Test PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MEGF8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MEGF8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MEGF8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MEGF8 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt IDQ7Z7M0

Standard Curve

Standard Curve标准曲线图
For Research Use Only. Not for Diagnostic Procedures.

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