人新型冠状病毒N蛋白酶联免疫吸附检测试剂盒
- Cat. No.：ECV001
- Price：Negotiable
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Product Detail
Product Information
| Product Name | Human SARS-CoV-2 Nucleocapsid ELISA Kit |
|---|---|
| Alternative Names | COVID-19;SARS-CoV-2; 2019-nCoV |
Assay Parameters
| Species | Human |
|---|---|
| Assay Type | Sandwich |
| Sensitivity | 3.2 pg/mL |
| Detection Range | 7.82-500 pg/mL |
| Sample Type | serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Assay Length | 3.5h |
Research Information
| Research Area | Infectious Diseases |
|---|---|
| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SARS-CoV-2 Nucleocapsid. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SARS-CoV-2 Nucleocapsid. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SARS-CoV-2 Nucleocapsid, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SARS-CoV-2 Nucleocapsid in the samples is then determined by comparing the OD of the samples to the standard curve. |
Standard Curve
| Standard Curve |  |
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